Novel and new records published by T.E.S.R.I.

Amanita ovalispora Boedijn, Sydowia 5: 320, 1951.

 Pileus convex to plano-convex, margin sulcato-striate, nearly halfway the cap, 5-7 cm in diam., smooth greyish brown. Flesh very thin, white, 1-2 mm thick near the stalk, rapidly thinning out near the margin. Gills free, pure white to cream colour, whitish with occasional pale yellow tint, moderately crowded, 4-5 mm high in the middle. Stem hollow, pure white to grayish white, 7-10 × 0.6-1.0 cm, at first covered by slightly flocculose or small adpressed scales, especially near the base, soon becoming glabrous, attenuated near the apex and not bulbous at the base. Wall of stem about 2 mm broad. Volva free, sheathing the base of the stem, dirty white, with lobed margin, 2.5-3 cm high, 1.5-2 cm broad. Pileipellis a trichodermium of septate hyphae. Basidia 43-57 × 12-18 mm, clavate, 4- spored, sterigmata 8 mm in NH₄OH seen to have numerous amorphous refractive yellowish particles scattered, basal septa without clamp. Basidiospores (8.0-)9.0-11.0(-12.0) ×(7.0-)7.5-9.0(-10.0) mm, Q = 1.12-1.38, broad elliptical to elliptical (Fig.1c), inamyloid, hyaline, smooth, thin-walled. Gill trama bilateral; mediostram 20-35 mm broad, composed of many longitudinal, elliptical to spindle-form inflated cells, 35-80 × 12-20 mm, interwoven with plenty of cylindrical branched hyphae, 2-5 mm broad, clamp connection absent; subhymenium 30-40 mm thick, inflated-ramose type, composed of 2-3 layers cells, inflated cells short elliptical to barrel-shaped (Fig. 2c). Volva surface layer composed of longitudinally filamentous hyphae, 2-10 mm broad; inner layer composed of dense, hyaline, cylindrical hyphae, 4-10 mm broad, sometimes intercalary inflated, up to 18 mm diam., while the closest layer to stem composed of loose, hyaline, cylindrical hyphae, 3-8 mm broad, inflated cells usually oval to subglose, up to 55-85 × 43-62 mm, with elliptical inflated cells, up to 60-120 × 30-57 mm, single and terminal. Stem trama largely composed longitudinally cylindrical hyphae, up to 250-350 × 23-40 mm, terminal clavate, interwoven filamentous hyphae, 4-8 mm diam. Cheilocystidia sterile, often composed of pyriform to broad clavate, with ovate to short elliptical (25-35 × 20-24 mm), thin-walled, hyaline inflated cells.        .
Specimen examined：Nantou, Sun Moon Lake, alt. 800m, H.W. Huang 789 ( 8.IX. 1994.); C.M. Chen 2413 (12.VII. 2000.).

Habitat：Solitary under broad-leaved trees.
Distribution：Taiwan, China, Indonesia.
Remark: The distinguishing characters of A. ovalispora are its free volva, hollow  stem and the convex to plano-convex pileus with sulcato-striate margin, which extends to nearly halfway of the cap. Lamellae are gray-brownish in color when dried, a distinct characters for the species.

Austroboletus dictyotus（Boedijn）Corner, Boletus in Malaysia  p.80 –81. 1972;    ≡Porphyrellus dictyotus Boedijn, Persoonia 1: 316. 1960.

  Pileus 5 -7 cm broad, conical, then convex and umbonate when age, floccoso- felted to subsquamulose, whitish then pale fawn, at centre especially . Surface of the pileus covered by a thick pile about I mm high, composed of long sparingly branched, cylindric hyphae 4-9 um wide, long-celled, often arising from shorter wider cells 18 um wide in the tissue adjoining the flesh of the pileus.  Context whitish, spongy, 8 - 10 mm thick in the centre of the pileus, unchanging when cut. Tubes 7 – 9 mm long,  strongly sinuate decurrent, decurrent diminishing in length near the stipe and the margin whitish then vinaceous pink or pale vinaceous cinnamon. Pores 0.8 – 1 mm wide, circular, concolorous with tubes. Stipe cylindrical 5.5-6 cm long, 14-16 mm broad, alveolate-lacunose, the alveolar 3-9 mm long, dirty yellow, about Chamois ( R).       Spore print deep rrufous madder to light chocolate. Spores 23 – 28 x 11 – 12.5 um, amygdaliform , exosporium ornamented coarsely tuberculate, 1.5 – 2 um deep, the ends pitting and pale, apiculus 0.3 um. Basidia clavate, 4-spored, 40-50 x 18-22.5 um; sterigmata conical, 7-8 um long, 1-2 um broad at the base, some with yellow content in KOH and Melzer,s. Pleurocystidia 71 – 132 x 20 – 26 um, mostly fusoid-ventricose, the apical half often elongation with one or two septa, 36- 61 um long, and the apex subacute. Cheilocystidia rare, size and shape similar to pleurocystidia.  Caulohymenium present over the whole stipe mainly consisting of basidioliform cells that are intermixed with both basidia and  polymorphic, cystidia – like elements, extra- and intracellular pigments in 3 % KOH vivid orange – brown to ochraceous in the lower part of the stipe, pale yellowish above. Clamp connection absent in all tissues which are non – amyloid. Pileipellis a trichodermium of loosely tangled tubular hyphae up to 200 um long, 5-12.5 um broad, walls more or less gelatinized, encrusted with brown to brownish yellow granules or patches dissolving in KOH, contents hyaline or brownish orange in Melzer,s in some element cells. Stipitipellis a continuous hymeniform layer, 25-35 um thick, consisting of sterile clavate cells, intermixed with caulobasidia and caulocystidia; caulobasidia usually 2-spored, scattered or occasional; caulocystidia 30-140 x 10-20 um, ventricose-fusoid to ventricose-appendiculate or subcylindric, often septate, thin-walled, contents hyaline to yellowish in KOH. Clamp connections absent.

Corner (1972) substituted subgen. Austroboletus for sect. Graciles (Singer) that was classified to genus Porphyrellus previously. Nevertheless Austroboletus resembles Strobilomyces in spore, Boletellus in pileus, and Heimiella in tube-trama. Thus Wolf (1979) proposed genus Austroboletus, based on the result of EM scanning observation. Its fruit bodies have a well developed marginal veil, a long lacunose-reticulate stem and boletoid tube-trama. Its spores are vinaceous pink to purple brown or chocolate in the mass; they are fusiform-amygdaloid and show a certain amount of compresion. They have a hyaline exospore and coloured endospore with conical warts or a close reticulation, apiculus 0.3-0.5 um long. Corner (1972) has explained that smooth boletoid spore has evolved from the ornamented one and Austroboletus is a transition between the twos. In our specimen examined, this collection can be easily distinguished from A. gracilis and A. subvirens reported before ( Chen et al., 1997) by the white color of pileus. In addition, there are major differences in basidiospore morphology among them. Basidiospores of A. dictyotus are reticulate – ruminate medial surface, foveate proximal and distal surface. While those of A. gracilis have a verrucose – foveate surface thoroughly, some of which are easily lost and we found it difficult to find ornamented spores (Fig. ). Basidiospores of A subvirens with wall becoming minutely pitted, eventually dissected by meandering subreticulate channels that occasionally isolate irregular truncate warts or ridges, especially around middle, with walls up to 1.4 um thick.. However, the acute distal end of the spores of A. dictyotus and the blunt distal end of the spores of A. subvirens can make it remarkable.

Habitat: Solitary in the forest.

Distribution: Taiwan, Malaysia, Indonesia.

Nantou: Shanlihsi, alt. 1750m, Nov. 23, 1998. Chen Chien-Ming（2303）.

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